Shared Genomics Tools
DEPARTMENT OF BIOLOGY CORE FACILITY
shRNA Tools
shRNA tools enable targeted gene knockdown through RNA interference (RNAi) allowing researchers to reduce expression of specific genes in mammalian cells, providing a powerful approach for studying gene function, identifying therapeutic targets, and validating signaling pathways. For a comprehensive collection of validated shRNA plasmids and resources, see Addgene Mammalian shRNA Tools for RNAi, MISSION® Lentiviral shRNA and General shRNA FAQs.
Lentiviral technology
Lentiviral vectors are versatile tools for delivering genetic material into a wide range of cell types. They support applications such as stable gene expression, CRISPR/Cas9-mediated genome editing, and CAR-T cell engineering. For detailed guidance, refer to Lentiviral Vector Guide, Lentiviral Plasmids, Lentivirus Production Protocols and CRISPR Guide.
Key videos - step-by-step explanations of lentiviral systems and their applications
- The Basics of the Recombinant Lentivirus System – fundamental concepts and workflow.
- Lentiviruses: A Versatile Tool for Cell Biology Research – overview of diverse applications.
- The Basics of Lentivirus Production/Packaging – practical tips and protocols.
- Lentivirus Transduction Protocol – efficient methods for infecting target cells.
- Lentivirus Titration - how to determine viral titer
Protocols
Standardized protocols are essential for reproducible results in plasmid preparation, lentivirus production, and transduction. Addgene offers detailed step-by-step protocols and instructional videos to guide researchers through these procedures: Addgene Protocols | Addgene Videos.
For detailed guidance for plasmid handling, see Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids. In addition, for obtaining a fresh single colony, see Streaking and Isolating Bacteria on an LB Agar Plate. And for preparing an overnight culture, see Inoculating a Liquid Bacterial Culture.
Laboratory Safety Guidelines
All laboratory work should follow Biosafety Level 1 (BSL‑1) or Biosafety Level 2 (BSL‑2) practices as appropriate for the material. This includes using proper PPE, maintaining aseptic techniques, and disinfecting work surfaces and waste. These guidelines apply to routine handling of plasmids, bacterial cultures, and lentiviral vectors. Refer to: Addgene Lab Safety for BSL‑1 and BSL‑2, Addgene Biosafety Resource Guide.
Other resources:
GPP web portal, Broad Institute